Review Outline for Exam II

Molecular Biology, Feb 23, 1998

1. How do you clone DNA into lambda phage? Why would you clone DNA into lambda phage instead of pBR322?

2. Compare and contrast a cosmid with a plasmid.

3. What is a genomic library? Why would you make such a library by using the products of a partial restriction enzyme digest?

4. What is the formula for determining how many colonies to screen in order to find your gene of interest?

5. What steps are involved in making a cDNA library?

6. What is a probe? What types of probes are commonly used in molecular biology labs?

7. What is a heterologous probe?

8. How could you find your gene of interest without using a nucleic acid-probe?

9. Outline the steps involved in making a subtraction library?

10. Cite a use for a Northern and Southern blot.

11. Distinguish between Northern, Southern, and Western blots.

12. List all the relevant steps involved in making either a Southern, Northern, or Western blot.

13. Explain the basis of the Sanger reaction.

14. Outline a method for introducing a single point mutation in your gene of interest.

15. Summarize the objectives and basic materials and methods used in the following labs: