1. Briefly explain the four levels of organization in protein structure?
2. What is a DNA melting curve and what does it tell us?
3. What is a Cot curve and what does it tell us?
4. Which molecular process is involved in genetic imprinting?
5. Why do you see two bands when plasmid DNA is run out on an agarose gel?
6. How do you isolate plasmid DNA from bacterial cells?
7. Describe the lysogenic cycle of bacteriophages?
8. What is a cos site?
9. T4 viruses express their genes for phage-head proteins after the phage DNA is replicated. How is this done?
10. In what way does the ampicillin-resistance gene on a plasmid facilitate transformation experiments?
11. EcoR1 makes a staggered cut with a 5 overhang. Draw this.
12. Why would you use alkaline phosphatase in an experiment?
13. Distinguish between selection and screening.
14. Map the EcoI, HindII, and PstI sites using the following data:
| Eco RI | 3.5, 2.5 kb frag |
| HindIII | 6 kb frag |
| PstI | 3.25, 2.75 |
| EcoRI/PstI | 2, 1.75, 1.5, 0.75 |
| HindIII/PstI | 2.75, 2, 1.25 |
| EcoRI/HindIII | 3.5, 2, 0.5 |
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15. Summarize the objectives and basic materials and methods used in the following labs: