Lecture Topics for Molecular
Biology
- DNA as the Molecule of Life
- DNA denaturation and melting curves
- DNA renaturation and Cot curves
- DNA methylation
- Genomic imprinting
- Bacterial structure and growth
- Plasmids - features/isolation/electrophoretic analysis
- Bacteriophages - features/life cycles/gene regulation/packaging of DNA
- Transformation
- Restriction enzymes
- Restriction site mapping
- Synthesis of recombinant DNA using pBR322 and pUC18 plasmids
- Cloning inserts into lambda DNA
- Cosmids
- Synthesis of genomic library
- Synthesis of cDNA library
- Screening libraries/probes
- Southern and Northern blot analysis
- DNA sequencing
- Site directed mutagenesis
- Eucaryotic RNA processing - polyadenylation and splicing
- Eucaryotic gene regulation - iron metabolism
- Eucaryotic gene structure - promoters/mechanism of Endo16
promoter/5'-deletion experiments/enhancers
- Transcription factors - mobility shift assays/DNA footprinting/motifs
- Eucaryotic gene organization - multigene families/pseudogenes
- Constructing transgenic mice and gene targeting